Ligand-Mediated Transcription Elongation Control Using Triplex-Based Padlock Oligonucleotides
نویسندگان
چکیده
منابع مشابه
Targeted inhibition of transcription elongation in cells mediated by triplex-forming oligonucleotides.
Triple-helix-forming oligonucleotides (TFOs) bind in the major groove of double-stranded DNA at oligopyrimidine small middle dotoligopurine sequences and therefore are candidate molecules for artificial gene regulation, in vitro and in vivo. We recently have described oligonucleotide analogues containing N3'-P5' phosphoramidate (np) linkages that exhibited efficient inhibition of transcription ...
متن کاملGene Control during Transcription Elongation
1Departamento de Genética, Facultad de Bioloǵıa, Universidad de Sevilla, Seville 41012, Spain 2Department of Biochemistry and Molecular Biology, Louisiana State University Health Sciences Center, Shreveport, LA 71130-3932, USA 3Laboratoire de Genetique Moleculaire, Academie Universitaire Louvain, 5000 Namur, Belgium 4Departamento de Bioloǵıa Molecular, Instituto de Parasitoloǵıa y Biomedicina “...
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Labeling of a covalently closed circular double-stranded DNA was achieved using a so-called 'padlock oligonucleotide'. The oligonucleotide was targeted to a sequence which is present in the replication origin of phage f1 and thus in numerous commonly used plasmids. After winding around the double-stranded target DNA sequence by ligand-induced triple helix formation, a biotinylated oligonucleoti...
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Triplex-forming oligonucleotides (TFOs) are generally designed to inhibit transcription or DNA replication but can be used for more diverse purposes. Here we have designed a hairpin-TFO able to recruit transcription factors to a target DNA. The designed oligonucleotide contains a triplex-forming sequence, linked through a nucleotide loop to a double-stranded hairpin including the SRE enhancer o...
متن کاملPadlock oligonucleotides for duplex DNA based on sequence-specific triple helix formation.
An oligonucleotide was circularized around double-stranded DNA thanks to triple helix formation. Short oligonucleotides are known to be able to form DNA triple helices by binding into the DNA major groove at an oligopurine.oligopyrimidine sequence. After sequence-specific recognition of a double-stranded DNA target through triple helix formation, the ends of the triplex-forming oligonucleotide ...
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ژورنال
عنوان ژورنال: Chemistry & Biology
سال: 2004
ISSN: 1074-5521
DOI: 10.1016/j.chembiol.2004.03.016